medparasitologyМедицинская паразитология и паразитарные болезниMedical Parasitology and Parasitic Diseases0025-83262713-1777Научно-образовательный паразитологический центр им. П.Г. Сергиева10.33092/0025-8326mp2025.4.28-36medparasitology-44Research ArticleОБЗОРREVIEWМетоды искусственного инфицирования иксодовых клещей in vitroMethods of artificial infection of ixodid ticks in vitroКоняеваО. А.KonyaevaO. A.

Ставрополь

Stavropol

МироненкоЕ. А.MironenkoE. A.

Ставрополь

Stavropol

КозубенкоЮ. С.KozubenkoYu. S.

Ставрополь

Stavropol

ВолынкинаА. С.VolynkinaA. S.

Ставрополь

Stavropol

КуличенкоА. Н.KulichenkoA. N.

Ставрополь

Stavropol

ФКУЗ Ставропольский противочумный институт РоспотребнадзораРоссияFKUZ Stavropol Anti-Plague Institute of the RospotrebnadzorRussian Federation202522042026042836Copyright © Научно-образовательный паразитологический центр им. П.Г. Сергиева, 20262026Научно-образовательный паразитологический центр им. П.Г. СергиеваНаучно-образовательный паразитологический центр им. П.Г. Сергиеваhttps://www.medparasitology.com/jour/about/submissions#copyrightNoticehttps://www.medparasitology.com/jour/article/view/44

Цели и задачи исследования проведение ретроспективного анализа научных работ по практическому применению методов искусственного кормления и инфицирования иксодовых клещей.

Материалы и методы

Материалы и методы. Проанализированы научные публикации из баз данных PubMed, eLIBRARY, Google Scholar и Scopus по методам искусственного инфицирования иксодовых клещей патогенами трансмиссивных инфекций in vitro. Оценены преимущества и ограничения каждого метода на основе опубликованных экспериментальных данных и статистики.

Результаты и обсуждение

Результаты и обсуждение. Статья рассматривает современные способы лабораторного инфицирования иксодовых клещей для изучения трансмиссивных заболеваний. Приведены ключевые методы — использование капилляров, мембран, инъекции и погружение клещей в растворы. Анализируются достоинства и недостатки методик, воздействие на жизнеспособность клещей и возможные направления улучшения технологий. Подчёркивается роль разработок, позволяющих отказаться от животных моделей и повысить точность эксперимента.

Objectives and tasks of the study: The study conducts a retrospective analysis of scientific publications regarding practical applications of methods for artificial feeding and infection of ixodid ticks.

Materials and Methods

Materials and Methods. Scientific articles were analyzed from databases such as PubMed, eLIBRARY, Google Scholar, and Scopus, focusing on in vitro methods of artificially infecting ixodid ticks with pathogens causing transmissible diseases. Advantages and limitations of each method are evaluated based on published experimental data and statistics.

Results and Discussion

Results and Discussion. This paper reviews contemporary laboratory techniques used for infecting ixodid ticks to investigate mechanisms of infectious disease spread. Key approaches include the use of capillaries, membranes, injection, and immersion of ticks into enriched solutions. Strengths and weaknesses of these methodologies are discussed along with their impact on tick viability and possible directions for improving technology. Special emphasis is placed on developments that reduce reliance on animal models while enhancing experimental accuracy.

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The authors declare that there are no conflicts of interest present.