Evaluation of the effectiveness of disinfectants with respect to multiresistant nosocomial ESKAPE group and parasitic pathogens in multidisciplinary hospitals

The problem of resistance to antibiotics and desinfectants forming in bacterial and parasitic pathogens is one of the most prominent in healthcare organisations. Circulation of multiresistant ESKAPE strains and contamination of hospital environment with parasitic pathogens leads to the need of constant monitoring and evaluation of effectiveness of the desinfection measures.

Aim of the study. This study was aimed at the evaluation of the sensitivity/resistance of bacterial isolates sampled from the environmental objects and biological material to antibiotics and desinfectants. Another subject of this work was to analyze the ovicidal antiparasitic activity of desinfectants routinely used in city hospitals in Moscow.

Materials and methods. Wipes and swabs samples (136) from the objects of hospital environment were analyzed. Bacteria identification was performed using mass-spectrometry method (MALDI-TOF MS). Sensitivity to desinfectants was assayed according to the regulatory documents. Sampling for parasitology analysis and ovicidal activity tests were conducted according to the regulatory documents and scientific research data.

Results. The following pathogens of ESKAPE group were achieved: 7 isolates of Staphylococcus aureus, Acinetobacter baumannii (5), Enterococcus faecium (1) and Klebsiella pneumoniae (11). Desinfectants “Omnika des”, “Omnika OFA”, “Torichlor” were effective against all studied isolates. “Omnika enzim” (at 0.5- 1% concentrations) demonstrated less impact on A. baumannii, Pseudomonas aeruginosa and K. pneumoniae; “Torioksi” — on K. pneumoniae; “Anolit” — on S. aureus (inhibition < 99, 99%). These desinfectants used in the inspected hospital demonstrated no ovicidal activity. Conclusion. The nosocomial circulation of multiresistant ESKAPE strains and parasitic contamination of hospital environment was revealed. The need for the rotation of the desinfectants (“Torioksi”, “Anolit”, “Omnika enzim”) with more effective alternatives was confirmed. New products (“Klindesin NUK 5”) were proven effective against the bacterial pathogens and suitable to be included in the desinfection schemes.

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