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Mass spectrometric database for identification of dirofilariae
https://doi.org/10.33092/0025-8326mp2023.4.11-15
EDN: AWADNI
Abstract
The results of using MS MALDI-TOF for diagnosing helminths are modest, but recent studies show the promise of this method for reliable identification of nematodes. The article provides information on compiling a database of dirofilaria (Dirofilaria immitis, Dirofilaria repens).
The protein spectra obtained from 58 D. repens and 118 D. immitis specimens were analyzed using a software mass spectrometer Bruker Daltonics Germany. Adult D. repens specimens and their fragments were obtained from 52 patients of medical organizations in the Rostov region and the South of Russia and from 6 dogs from veterinary clinics in the Rostov region. Adult D. immitis were removed from the heart and large pulmonary vessels of 11 animals of the canine family. The obtained numerical values determine the number of reference spectra and, on their basis, create databases for the identification of the nematode of the genus Dirofilaria. Type and version of the database management system: Bruker Daltonics Biotyper. Database size 562 KB (0,562 MB): D. repens - 145 KB, D. immitis - 417 KB. Based on the data obtained a certificate of registration.
About the Authors
I. S. Berezinskaya
Rostov Research Institute of Microbiology and Parasitology Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Russian Federation
Russian Federation
Rostov-on-Don
S. A. Nagorny
Rostov Research Institute of Microbiology and Parasitology Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Russian Federation
Russian Federation
Rostov-on-Don
A. V. Aleshukina
Rostov Research Institute of Microbiology and Parasitology Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Russian Federation
Russian Federation
Rostov-on-Don
L. A. Ermakova
Rostov Research Institute of Microbiology and Parasitology Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Russian Federation
Russian Federation
Rostov-on-Don
N. V. Chebyshev
FSAE Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
I. B. Martyusheva
Rostov Research Institute of Microbiology and Parasitology Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Russian Federation
Russian Federation
Rostov-on-Don
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Review
For citations:
Berezinskaya I.S., Nagorny S.A., Aleshukina A.V., Ermakova L.A., Chebyshev N.V., Martyusheva I.B. Mass spectrometric database for identification of dirofilariae. Medical Parasitology and Parasitic Diseases. 2023;(4):11-15. (In Russ.) https://doi.org/10.33092/0025-8326mp2023.4.11-15. EDN: AWADNI
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