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Metagenomic analysis of viromes of Aedes (Stegomyia) albopictus skus. mosquitoes оf the Black sea coast of the Caucasus
https://doi.org/10.33092/0025-8326mp2024.3.3-9
EDN: ZVGTUO
Abstract
The aim of the study was to identify viruses contained in female Ae. albopictus mosquitoes using de novo metagenomic sequencing. Mosquitoes were collected using standard entomological methods in August 2019 in the Kuban in Tuapse, Adler, on the territory of the Adler Monkey Nursery and in the city of Gagra, Abkhazia. Adults were identified by morphological characteristics. A total of 1,336 mosquitoes were caught. We formed four pools at the collection site, with 300 to 400 specimens in each. The virus was considered detected if a specific nucleotide sequence of at least 1000 bases was detected in the mosquito sample. A total of 8 insect viruses were identified. Four viruses are classified and belong to the families Rhabdoviridae, Orthomyxoviridae, Chuviridae. The families of the other four viruses have not yet been determined, but two viruses are classified in the order Bunyavirales. No viruses pathogenic for humans were detected.
About the Authors
S. V. Alkhovsky
N.F.Gamaleya Federal Research Centre of Epidemiology and Microbiology
Russian Federation
Russian Federation
Moscow
T. V. Vishnevskaya
N.F.Gamaleya Federal Research Centre of Epidemiology and Microbiology
Russian Federation
Russian Federation
Moscow
O. I. Vyshemirsky
Kurchatov Complex of Medical Primatology, National Research Center “Kurchatov Institute”
Russian Federation
Russian Federation
Sochi
L. A. Ganushkina
Sechenov First Moscow State Medical Univesity
Russian Federation
Russian Federation
Moscow
S. V. Lenshin
Sochi anti-plague department of the FKUZ Stavropol Anti-plague Institute Rospotrebnadzor
Russian Federation
Russian Federation
Stavropol
I. V. Patraman
Sechenov First Moscow State Medical Univesity
Russian Federation
Russian Federation
Moscow
N. V. Chebyshev
Sechenov First Moscow State Medical Univesity
Russian Federation
Russian Federation
Moscow
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For citations:
Alkhovsky S.V., Vishnevskaya T.V., Vyshemirsky O.I., Ganushkina L.A., Lenshin S.V., Patraman I.V., Chebyshev N.V. Metagenomic analysis of viromes of Aedes (Stegomyia) albopictus skus. mosquitoes оf the Black sea coast of the Caucasus. Medical Parasitology and Parasitic Diseases. 2024;(3):3-9. (In Russ.) https://doi.org/10.33092/0025-8326mp2024.3.3-9. EDN: ZVGTUO
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