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Determination of specific antibodies to Toxocara canis in humans by immunochromatographic analysis
https://doi.org/10.33092/0025-8326mp2026.21-28
Abstract
The article describes a method for obtaining immunoreagents for the serological diagnosis of human toxocariasis by immunochromatographic analysis. A test system has been developed for serological screening of the population for toxocariasis by an immunochemical method using excretory-secretory antigens of Toxocara larvae (patent No. 2834042 dated 03/16/2023 RU). Preliminary preparation of antigenic drug samples was carried out by optimizing the cultivation conditions of Toxocara canis larvae, followed by antigen production according to their own methodology. Highly immune sera as an immunoreagent were obtained from hyperimmunized animals with their own antigenic preparation in combination with Freund's adjuvant. When developing the test system, the purpose of screening was taken into account – the detection of antigens or antibodies in the test material. In order to test the immunochromatographic test system, blood serum samples from clinically healthy volunteers (n=154) were examined. According to the results, in 9 out of 109 (8.2%) cases among adults and in 4 out of 45 (8.8%) children's serum samples, antibodies to toxocars were visually observed in the form of transverse dark blue bands in the test zones. The specificity of the rapid test is confirmed by the similarity of the results of the ELISA study. The reproducibility of the rapid test demonstrates the accuracy of the methodology, the stability of the components used, and the possibility of its use in commercial production.
About the Authors
I. V. Kanina
Ryazan State Medical University named after academician I. P. Pavlov
Russian Federation
Russian Federation
Ryazan
M. D. Novak
Ryazan State Medical University named after academician I. P. Pavlov
Russian Federation
Russian Federation
Ryazan
A. I. Novak
Ryazan State Medical University named after academician I. P. Pavlov
Russian Federation
Russian Federation
Ryazan
O. V. Evdokimova
Ryazan State Medical University named after academician I. P. Pavlov
Russian Federation
Russian Federation
Ryazan
References
1. Davidyanc V.A., CHernikova E., Lungu V. Control and prevention of geohelminthiasis in the countries of the WHO European Region. A collection of reference and methodological materials. VOZ; 2017:5669. ISBN 978 92 890 5242 9.
2. Ma G., Holland C.V., Wang T. [et al.]. Human toxocariasis. Lancet Infect Dis. 2018;18(1):14–24. DOI: https://doi.org/10.1016/S1473-3099(17)30331-6.
3. Trocenko O.E., Ivanova I.B., Dragomerekaya A.G. [at al.]. Current issues of geohelminthiasis in the Russian Far East. Public health and habitat. 2016; 11(284): 37–40. EDN: WZTVCZ.
4. Patent RU 2834042. Rapid test for toxocariasis and methods for manufacturing components for its implementation. Applicant: Novak M.D., Evdokimova O.V., et al.; published 03.02.2025. - in Russian.
5. Bolatchiev K. H. Results of epizootological and epidemiological monitoring of toxocariasis in southern Russia. Russian Journal of Parasitology. 2019;13(4):17–24. DOI: https://doi.org/10.31016/1998-8435-2019-13-4-17-24.
6. Marcev A.A. Parasitic diseases of humans in the Vladimir region; 2023:137 s. ISBN 978-5-9984-1714-6.
7. O sostoyanii sanitarno-epidemiologicheskogo blagopoluchiya naseleniya v Rossijskoj Federacii v 2022 godu: gosudarstvennyj doklad. M.: Federal'naya sluzhba po nadzoru v sfere zashchity prav potrebitelej i blagopoluchiya cheloveka; 2023:368 s. ISBN 978–5–7508–2012–2 - in Russian.
8. On the state of sanitary and epidemiological well-being of the population in Khabarovsk Krai in 2021: state report. Khabarovsk: Office of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing for Khabarovsk Krai; 2022:88 - in Russian.
9. Khromenkova E. P. Sanitary and helminthological substantiation of environmental protection measures as a basis for the prevention of helminthiasis; 1992:16.; 1992:16. EDN: ZLQVER.
10. Khromenkova E. P., Tverdokhlebova T. I., Dimidova L. L. The importance of parasitological safety criteria for environmental objects during sanitary and parasitological monitoring. Far Eastern Journal of Infectious Pathology. 2015;29:87–90. - in Russian.
11. Konanykhina S. Yu. Clinical and immunological aspects of the diagnosis of toxocariasis in children; 2004:168 - in Russian.
12. Maslyaninova A.E., Shandrigos Yu. D., Mazurina E.O. [et al.]. Clinical and epidemiological aspects of human toxocariasis. International Research Journal. 2023; 11(137).DOI: https://doi.org/10.23670/IRJ.2023.137.107
13. Krasnov V. M. Toxocariasis. Modern approaches to diagnosis and treatment. Selected lectures on pediatrics; 2001: 75–83 DOI: https://doi.org/10.23670/IRJ.2023.137.107.
14. Sizhazheva A. M., Khulaev I. V., Shogenova R. S. [et al.]. Laboratory diagnostics of parasitic infections using modern research methods. Advances in modern science. 2017;2(2):185–9 2017;2(2):185–9.
15. Ermakova L. A., Tverdokhlebova T. I., Pshenichnaya N. Yu. Diagnostic value of enzyme immunoassay in larval helminthiases (trichinellosis, echinococcosis, toxocariasis). Preventive and clinical medicine. 2012;3(44):59– 63.EDN: PUXHIL.
16. Zakharchuk A. I., Bodnya E. I. Immunodiagnostics of toxocariasis. Parasitic diseases of humans, animals, and plants; 2008:90–9.
17. Smirnova D. N., Krupina K. A., Bogacheva N. V., Darmov I. V. Comparative evaluation of components of immunochromatographic test systems used for their development. Clinical laboratory diagnostics. 2017;62(1):30- 4.2017;62(1):30-4.DOI: http://dx.doi.org/10.18821/0869-2084-2017-62-1-30-34.
18. Novak M.D., Novak A.I., Kanina I.V. Development and application of immunoreagents for seroepizootological monitoring. International research journal. 2022;1(115):47-50. DOI: http://dx.doi.org/10.23670/IRJ.2022.115.1.049.
19. Novak M.D., Novak A.I., Kanina I.V. Application of the immunochromatographic method for the diagnosis of toxoplasmosis and toxocariasis. Theory and practice of combating parasitic diseases. 2023;24:337-42. DOI: http://dx.doi.org/10.31016/978-5-6048555-6-0.2023.24.337-342.
20. Kanina I.V., Novak A.I., Evdokimova O.V. [et al.]. Production of immunodiagnostic preparations from Toxocara canis antigens for serodiagnosis of toxocariasis in humans. Infection and Immunity. 2022;12(2):391–6.
21. Długosz E., Wiśniewski M. Toxocara canis glycans influence antigen recognition by mouse IgG1 and IgM antibodies. Acta Parasitol. 2016;61(1):191-4. DOI: http://dx.doi.org/10.1515/ap-2016-0026.
22. Fan C.K. Pathogenesis of cerebral toxocariasis and neurodegenerative diseases. Adv Parasitol. 2020;109:233-59. DOI:http://dx.doi.org/10.1016/bs.apar.2020.01.008.
23. Ma G., Rostami А., Wang T. [et al.]. Global and regional seroprevalence estimates for human toxocariasis: A call for. Adv Parasitol. 2020;109:275-90. DOI: http://dx.doi.org/10.1016/bs.apar.2020.01.011.
24. Badley J.E., Grieve R.B., Rockey J.H. [et al.]. Immune-mediated adherence of eosinophils to Toxocara canis infective larvae: the role of excretory-secretory antigens. Parasite Immunol.1987;9(1):133-43. DOI: http://dx.doi.org/10.1111/j.1365-3024.1987.tb00494.x.
25. Menu E., Kopec L., Luciani L. [et al.]. Measurement of the IgG Avidity Index in the Diagnosis of Clinical Toxocariasis Patients. Pathogens.2021;10(9):108693.DOI:http://dx.doi.org/10.3390/pathogens10091086.
26. Harris N.L, Loke Р. Recent Advances in Type-2-Cell-Mediated Immunity: Insights from Helminth Infection. Immunity. 2017;47(6):1024- 36.DOI:http://dx.doi.org/10.1016/j.immuni.2017.11.015.
27. Noordin R., Yunus M.H., Tan Farrizam S.N. [et al.]. Serodiagnostic methods for diagnosing larval toxocariasis. Adv Parasitol. 2020;109:131-52. DOI: http://dx.doi.org/10.1016/bs.apar.2020.01.003.
28. Morales-Ya´nez F., Marie T.S., Idalia H. [et al.]. Ultrasensitive detection of Toxocara canis excretory secretory antigens by a nanobody electrochemical magnetosensor assay. Anal Chem. 2019;91(18):11582-88. DOI:http://dx.doi. org/10.1021/acs.analchem.9b01687.
Review
For citations:
Kanina I.V., Novak M.D., Novak A.I., Evdokimova O.V. Determination of specific antibodies to Toxocara canis in humans by immunochromatographic analysis. Medical Parasitology and Parasitic Diseases. 2026;(1):21-28. (In Russ.) https://doi.org/10.33092/0025-8326mp2026.21-28
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