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Comparison of the effectiveness of two indirect methods of laboratory diagnosis of giardiasis
https://doi.org/10.33092/0025-8326mp2026.1.29-35
Abstract
The effectiveness of ICT and ELISA methods for the diagnosis of giardiasis was compared. 136 stool samples were examined to determine the sensitivity and specificity of the tests. FES was used as a reference method. The quick and easy-to-perform ICT showed a sensitivity of 100% and a specificity of 99.13%. The low specificity of the Vector-Best ELISA test, which was 100% and 12%, respectively, makes it unsuitable even for screening studies.
About the Authors
M. S. Maksimova
FSAE Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
L. V. Fedyanina
FSAE Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
E. V. Stepanova
FSAE Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
К. G. Ivanova
FSAE Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
A. A. Dmitriev
FSAE Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
A. V. Kondrashin
FSAE Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
M. F. Latypova
State Виdgetary Institution «Research Institute for Healthcare Organization and Medical Management of Moscow Healthcare Departament»
Russian Federation
Russian Federation
E. M. Shabanova
FSAE Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
E. S. Petrova
FSAE Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russian Federation
Russian Federation
Russian Federation
References
1. Sergiev V.P., Lobzin Yu.V., Kozlov S.S. Parasitic diseases of humans: (protozoan and helminthic diseases): Manual for doctors. St.Petersburg: OOO Izdatelstvo Foliant, 2016: 640 (in Russian).
2. Tumolskaya N.I. The role of Giardia in human pathology // Sechenov Medical Journal. 2014; 4(18): 54-64 (in Russian).
3. Karpov S.A. Giardia // Great Russian Encyclopedia: scientific and educational portal. 2022. (in Russian).
4. Giardiasis in children and adults: Clinical guidelines. Moscow: Ministry of Health of the Russian Federation, 2024. (in Russian).
5. MU 3.2.3966-23. Prevention of parasitic diseases. Epidemiological surveillance and prevention of giardiasis: Methodological guidelines. Moscow: Federal Center for Hygiene and Epidemiology of Rospotrebnadzor, 2023. (in Russian).
6. Istre G.R., Dunlop T.S., Gaspard G.B., Hopkins R.S. Waterborne giardiasis at a mountain resort: evidence for acquired immunity // American Journal of Public Health. 1984; 74(6): 602-604. DOI: 10.2105/AJPH.74.6.602
7. Bronshtein A.M. Tropical diseases and travel medicine. Moscow: GEOTAR-Media, 2014: 124 (in Russian).
8. Bronshtein A.M., Tokmalaev A.K. Parasitic diseases of humans: protozooses and helminthiases. Moscow: RUDN Publishing House, 2002: 207 (in Russian).
9. Ryan U., Zahedi A. Molecular epidemiology of giardiasis from a veterinary perspective // Advances in Parasitology. 2019; 106: 209-254. DOI: 10.1016/bs.apar.2019.07.002
10. Messa A. Jr., Köster P.C., Garrine M., Gilchrist C., Bartelt L.A. et al. Molecular diversity of Giardia duodenalis in children under 5 years from the Manhiça district, Southern Mozambique // PLoS Neglected Tropical Diseases. 2021; 15(1): e0008987. DOI: 10.1371/journal.pntd.0008987
11. On the state of sanitary and epidemiological welfare of the population in the Russian Federation in 2023: State Report. Moscow: Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, 2024: 364 (in Russian).
12. MUK 4.2.3145-13. Parasitological methods of laboratory diagnostics of helminthiases and protozooses: Methodological guidelines. Moscow: Federal Center for Hygiene and Epidemiology of Rospotrebnadzor, 2014: 72 (in Russian).
13. Gutiérrez-Cisneros M.J., MartínezRuiz R., Subirats M., Merino F.J., Millán R., Fuentes I. Assessment of two commercially available immunochromatographic assays for a rapid diagnosis of Giardia duodenalis and Cryptosporidium spp. in human fecal specimens // Enfermedades Infecciosas y Microbiología Clínica. 2011; 29(3): 201-203. DOI: 10.1016/j.eimc.2010.09.005
14. Nikolaeva I.V. Modern principles of diagnosis and treatment of giardiasis in children // Practical Medicine. 2014; 7(83): 17-22 (in Russian).
15. Bekhtereva M.K., Luppova N.E., Kornienko E.A., Minina S.N. et al. Working protocol for the diagnosis and treatment of giardiasis in children // Proceedings of the XX Congress of Pediatric Gastroenterologists of Russia and CIS Countries. Moscow, March 19-21, 2013. Moscow: 2013: 72- 77 (in Russian).
16. Shumanskaya S.Yu., Grigorieva E.E., Dronina A.M., Fomina E.G. Giardiasis. A modern view of the problem // News of Biomedical Sciences. 2023; 23(2): 90-97 (in Russian).
17. Alshinbekova G.K., Busova T.V., Auesbekova Zh.A. et al. Clinical manifestations, diagnosis and treatment of giardiasis in young children // Issues of Sustainable Development of Society. 2022; 9: 414-423 (in Russian).
Review
For citations:
Maksimova M.S., Fedyanina L.V., Stepanova E.V., Ivanova К.G., Dmitriev A.A., Kondrashin A.V., Latypova M.F., Shabanova E.M., Petrova E.S. Comparison of the effectiveness of two indirect methods of laboratory diagnosis of giardiasis. Medical Parasitology and Parasitic Diseases. 2026;(1):29-35. (In Russ.) https://doi.org/10.33092/0025-8326mp2026.1.29-35
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