Study of the mechanisms of detoxification of proinsecticides in the german cockroach Blattella germanica

The data on the observed detoxification process of the proinsecticides indoxacarb and chlorfenapyr in the body of the German cockroach, Blattella germanica of several laboratory strains are presented. Different resistance ratios to indoxacarb of M10 (16.7×), DM (45.8×) and MAG (10.4×) strains, and chlorfenapyr (6.7×, 2.0×, 1.7×, respectively) against the background of high resistance to cypermethrin (92–833×), fipronil (46–192×), tolerance to chlorpyrifos (5.0–50×) and thiamethoxam (3.5–10×). The insecticidal activity of indoxacarb and chlorfenapyr was studied with desynchronized application of synergists PPB, TBTP, DEM - inhibitors of insect enzyme systems and an inhibitor of ABC transporters verapamil. The antagonistic action of synergists indicates the difficulty of converting proinsecticides into a toxic metabolite. In the M10 strain, PPB and TBTP significantly suppressed the activity of chlorfenapyr, while in DM and MAG strains, activity of chlorfenapyr suppressed only under the action of TBTP. For the DM strain most resistant to indoxacarb, the use of TBTP and DEM reduces resistance by 2.8 and 2.4 times, respectively. Verapamil has a statistically significant effect on susceptibility to chlorfenapyr only in S-NIID and DM strains, and to indoxacarb in MAG and DM strains. 

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