Following entomological monitoring of blood-sucking mosquitoes, two species - Aedes albopictus and Culex pipiens - were morphologically identified. The invasive mosquito Ae. albopictus was first detected in Dagestan in the summer of 2025. To accurately identify Ae. albopictus, the sequence of the internal transcribed spacer of the rRNA gene cluster was determined. DNA analysis of the mosquitoes detected in Makhachkala and Derbent revealed that the DNA of Ae. albopictus from Krasnodar region was identical. Ae. albopictus mosquitoes are proven carriers of many arboviral infections, and their presence in the region poses a risk of developing local outbreaks. Dagestan, due to its climatic characteristics, is a favorable region of Russia for Ae. albopictus mosquitoes. The results of the study demonstrate the expansion of the range of invasive Ae. albopictus mosquitoes across Russia.

This article presents a retrospective analysis of the epidemiological situation of the invasion of parasitic diseases of the population, and environmental objects in the territory of the Karachay-Cherkess Republic from 2014 to 2024, describes the main problems of monitoring parasitosis.

The prevalence of arctic fox (Vulpes lagopus beringensis) trichinellosis on Bering Island is 36.4%, which indicates the presence of an active local isolated focus of a natural type. The average amount is about 90 larvae/g in the studied muscle groups of the hind limbs. The presence of numerous larvae in the samples at different stages of capsule formation indicates an active infection of the animals. No Trichinella larvae were found in the muscle samples of the American mink. The study was conducted under the state assignment of Lomonosov Moscow State University.

The article describes a method for obtaining immunoreagents for the serological diagnosis of human toxocariasis by immunochromatographic analysis. A test system has been developed for serological screening of the population for toxocariasis by an immunochemical method using excretory-secretory antigens of Toxocara larvae (patent No. 2834042 dated 03/16/2023 RU). Preliminary preparation of antigenic drug samples was carried out by optimizing the cultivation conditions of Toxocara canis larvae, followed by antigen production according to their own methodology. Highly immune sera as an immunoreagent were obtained from hyperimmunized animals with their own antigenic preparation in combination with Freund's adjuvant. When developing the test system, the purpose of screening was taken into account – the detection of antigens or antibodies in the test material. In order to test the immunochromatographic test system, blood serum samples from clinically healthy volunteers (n=154) were examined. According to the results, in 9 out of 109 (8.2%) cases among adults and in 4 out of 45 (8.8%) children's serum samples, antibodies to toxocars were visually observed in the form of transverse dark blue bands in the test zones. The specificity of the rapid test is confirmed by the similarity of the results of the ELISA study. The reproducibility of the rapid test demonstrates the accuracy of the methodology, the stability of the components used, and the possibility of its use in commercial production.

The effectiveness of ICT and ELISA methods for the diagnosis of giardiasis was compared. 136 stool samples were examined to determine the sensitivity and specificity of the tests. FES was used as a reference method. The quick and easy-to-perform ICT showed a sensitivity of 100% and a specificity of 99.13%. The low specificity of the Vector-Best ELISA test, which was 100% and 12%, respectively, makes it unsuitable even for screening studies.

Introduction. The article provides a general overview of viral diseases of farm animals transmitted through blood-sucking arthropods. The causative agents of diseases such as nodular nodular dermatitis, bluetongue, equine infectious anemia, West Nile fever, and tick-borne encephalitis are considered. The epizootic situation for each pathogen, methods of modern diagnosis and prevention of these diseases are briefly considered.

The implementation of the action plan for the development and strengthening of the federal state sanitary and epidemiological supervision system for 2021-2028 includes the priority tasks of strengthening the infrastructure and resource provision of the laboratory base of the bodies and organizations carrying out sanitary and epidemiological supervision, and improving the efficiency and quality of the analysis and forecast of the sanitary and epidemiological situation; improving the efficiency (speed) of responding to threats to the sanitary and epidemiological well-being of the population (Order of the Government of the Russian Federation No. 3680-r dated December 30, 2020).

The purpose of the study. The organization of laboratory control as part of the activities of the departmental service of state sanitary and epidemiological supervision to ensure a stable sanitary and epidemiological situation and biosafety of subordinate facilities.a stable sanitary and epidemiological situation and biosecurity at subordinate facilities.

Materials and methods. The analysis of sectoral statistical data "Information on the activities of laboratories of the sanitary-hygienic and microbiological profile of Federal budgetary healthcare institutions - Centers of Hygiene and Epidemiology" 20 regulatory legal acts and literary sources (electronic databases gov.ru, discat. ru) were analyzed, and more, than 831000 laboratory tests were performed. Methods of statistical and analytical analysis and generalization of data from basic monitoring observations were applied, and the effectiveness of the laboratory service of the departmental institution of state sanitary and epidemiological supervision was assessed.

Research results. Over the analyzed period, the overall indicators of laboratory research have been stabilized at the level of the estimated average annual volumes, and there is a positive trend over the years of observation, with an increase of 1.1 times for sanitary bacteriological research, more than 18.4 times for sanitary virological research, 1.7 times for sanitary parasitological research, and 4.4 times for research on particularly dangerous infections.

Conclusion. With the current trends in the implementation of a laboratory control complex that covers all types of microbiological research, the target indicator has been achieved: a positive trend in the effectiveness of the monitoring and laboratory control system for biological safety at subordinate facilities, which allows for a positive assessment of their results as socially significant indicators in the activities of the Center for State Sanitary and Epidemiological Surveillance of the Department of the President of the Russian Federation.

The article provides a general overview of viral diseases of farm animals transmitted through blood-sucking arthropods. The causative agents of diseases such as nodular dermatitis, bluetongue, equine infectious anemia, West Nile fever, and tick-borne encephalitis are considered. The epizootic situation for each pathogen, methods of modern diagnosis and prevention of these diseases are briefly considered.

Consumption of raw or inadequately processed marine or migratory fish may result in anisakidosis – a zoonotic disease caused by larvae of the parasitic nematodes of the family Anisakidae. In this study, we report the case of gastric anisakiasis in Vladivostok, in a 21-year-old male patient, after eating lightly salted chum salmon (Oncorhynchus keta). The parasite was identified as the third-stage larva of Anisakis sp. on the basis of morphology. The issues of prevention of anisakids infection are discussed.

The article describes a case of extensive anisakids infestation of an exotic bird, a female Leptoptilos dubius, kept in a private zoo in the Moscow region.

The problem of resistance to antibiotics and desinfectants forming in bacterial and parasitic pathogens is one of the most prominent in healthcare organisations. Circulation of multiresistant ESKAPE strains and contamination of hospital environment with parasitic pathogens leads to the need of constant monitoring and evaluation of effectiveness of the desinfection measures.

Aim of the study. This study was aimed at the evaluation of the sensitivity/resistance of bacterial isolates sampled from the environmental objects and biological material to antibiotics and desinfectants. Another subject of this work was to analyze the ovicidal antiparasitic activity of desinfectants routinely used in city hospitals in Moscow.

Materials and methods. Wipes and swabs samples (136) from the objects of hospital environment were analyzed. Bacteria identification was performed using mass-spectrometry method (MALDI-TOF MS). Sensitivity to desinfectants was assayed according to the regulatory documents. Sampling for parasitology analysis and ovicidal activity tests were conducted according to the regulatory documents and scientific research data.

Results. The following pathogens of ESKAPE group were achieved: 7 isolates of Staphylococcus aureus, Acinetobacter baumannii (5), Enterococcus faecium (1) and Klebsiella pneumoniae (11). Desinfectants “Omnika des”, “Omnika OFA”, “Torichlor” were effective against all studied isolates. “Omnika enzim” (at 0.5- 1% concentrations) demonstrated less impact on A. baumannii, Pseudomonas aeruginosa and K. pneumoniae; “Torioksi” — on K. pneumoniae; “Anolit” — on S. aureus (inhibition < 99, 99%). These desinfectants used in the inspected hospital demonstrated no ovicidal activity. Conclusion. The nosocomial circulation of multiresistant ESKAPE strains and parasitic contamination of hospital environment was revealed. The need for the rotation of the desinfectants (“Torioksi”, “Anolit”, “Omnika enzim”) with more effective alternatives was confirmed. New products (“Klindesin NUK 5”) were proven effective against the bacterial pathogens and suitable to be included in the desinfection schemes.