One of the tasks of epidemiological surveillance of parasitic diseases is to maintain sanitary and epidemiological well-being for endemic biohelminthiasis in the territories included in the range of pathogens. The inclusion of a person in the circulation of pathogens depends on a complex of social factors, primarily on the nutritional characteristics of residents. Long-term studies show that the official registration data do not fully reflect the real picture of the population affected by pathogens of biohelminthiasis. Sanitary and parasitological examination of fish occupies one of the leading places in the system of preventive measures. The main task at the present stage is to provide a differentiated approach to the development and optimization of means and methods of disease prevention among the population of endemic territories, depending on the type of focus, the level of endemia and, accordingly, the degree of risk of infection of the population.

The results of using MS MALDI-TOF for diagnosing helminths are modest, but recent studies show the promise of this method for reliable identification of nematodes. The article provides information on compiling a database of dirofilaria (Dirofilaria immitis, Dirofilaria repens).
The protein spectra obtained from 58 D. repens and 118 D. immitis specimens were analyzed using a software mass spectrometer Bruker Daltonics Germany. Adult D. repens specimens and their fragments were obtained from 52 patients of medical organizations in the Rostov region and the South of Russia and from 6 dogs from veterinary clinics in the Rostov region. Adult D. immitis were removed from the heart and large pulmonary vessels of 11 animals of the canine family. The obtained numerical values determine the number of reference spectra and, on their basis, create databases for the identification of the nematode of the genus Dirofilaria. Type and version of the database management system: Bruker Daltonics Biotyper. Database size 562 KB (0,562 MB): D. repens - 145 KB, D. immitis - 417 KB. Based on the data obtained a certificate of registration.

The importance of blood-sucking mosquitoes as carriers of dirofilariasis pathogens in the city of Omsk and in suburban areas is shown. Candidates for the role of dirofillariasis vectors in in these territories, belonging to the genera Aedes, Ochlerotatus, Culex, Anopheles, have been identified. Their calculated infestation was determined. The periods of risk of infection were calculated for species emerging in the second decade of May (Ae.cinereus, O.flavescens, An.messeae) and species emerging closer to the end of May (O. dorsalis, O. excrucians).

To assess the influence of genetic features of metabolic processes on the reactivity to opisthorchiasis invasion, biochemical parameters of the hepatobiliary system functions were studied in chronic opisthorchiasis patients with and without mutant alleles of polymorphisms associated with lipid metabolism disorders were studied. It was found that the presence of mutations in the loci rs2854117 APOC3, rs7412 APOE enhances the negative impact of chronic opisthorchiasis invasion on the state and function of hepatocytes. The presence of the rare allele rs11206510 PCSK9 prevents an increase in ALP activity. In subgroups with mutations in individual polymorphisms of the APOE, APOC3I, ABCA1, lipoprotein lipase, and paraoxanase-1 genes, differences were found in the change in parameters after anthelmintic therapy, possibly associated with their role in the response of metabolic processes not only to invasion, but also to the treatment process.

The purpose of our study was to conduct a comparative analysis of two methods of semi-quantitative accounting of the results of enzyme immunoassay: calculation by "sample index" and by "antibody units". For a comparative assessment of the accounting method, 111 blood serum samples of patients with a positive result for opisthorchiasis were taken. Diagnosis of opisthorchiasis according to the clinical picture of the disease is difficult due to the absence of symptoms and syndromes characteristic only of this disease. When using laboratory diagnostic methods, it is necessary to correctly select the method of accounting for the results obtained.

Due to the peculiarities of keeping and exploitation of service dogs, their frequent movements to various territories both within the country and to neighboring states, this group of animals is an important factor in expanding the range of dirofilariasis. This paper presents the results of a study of infection of service dogs with dirofilaria (Dirofilaria repens and Dirofilaria immitis) for the period from 2010 to 2022 from different territories of the Russian Federation: Rostov, Novgorod, Astrakhan, Orel, Tula, Belgorod, Bryansk and Kursk regions, Krasnodar and Stavropol territories, the Republics of Bashkortostan, Kalmykia and Adygea. 948 blood samples of dogs were studied, microfilariae were detected in 140 samples, in general, the prevalence of invasion was 14,8 ± 1,1 %. D. repens microfilariae were found in 11,0 ± 1,0 % of dogs, D. immitis 2,1 ± 0,4 %, simultaneously both types of microfilariae (mixed invasion) 1,4 ± 0,4 %.
Analysis of the data obtained indicates a high prevalence of service dogs with this biohelminthiasis. In recent years, there has been a decrease in the incidence among service dogs in the study areas, which was facilitated by active preventive measures: improved diagnosis of dirofilariasis, the use of effective microfilariacid preparations, and vector control.

Practical experience in diagnosing infectious and parasitic diseases of poultry is presented using the example of an industrial flock of laying hens kept on the territory of a poultry farm. An assessment is made of the spectrum of pathogens of parasitic, bacterial and viral etiology circulating in a particular poultry farm. Clinical, pathological, parasitological and serological studies were carried out on samples of material taken from the poultry population. The immune status of the chicken population was monitored in relation to pathogens of infectious and parasitic diseases and the effectiveness of specific prevention against infectious and parasitic diseases in poultry was analyzed.

The article raises the question of the peculiarities of the prevalence of toxoplasmosis in the Arctic zone of the Russian Federation, in the wild of which there is no definitive host.

The presented scientific and analytical material presents the experience of training specialists who improve their qualifications in the field of sanitary parasitology for the subsequent application of the knowledge gained in practice in ensuring the epidemiological well-being of the state and the effective assessment of parasitic pollution of environmental objects.